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KMID : 0604520140400040365
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Journal of the Society of Cosmetic Scientists of Korea
2014 Volume.40 No. 4 p.365 ~ p.371
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Anti-Wrinkle Effect of Schizandra chinensis Baillon
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Lee Jung-Hee
Kim Jong-Im
Choi Hwa-Jung
Lee Jung-Hyun
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Abstract
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To identify new active anti-wrinkle ingredients, this study investigated the anti-wrinkle effects of Schizandra chinensis Baillon fermented with Lactobacillus plantarum (SCF) by assessment of cytotoxicity of human dermal fibroblast, collagen biosynthesis, matrix metalloproteinase-I (MMP-1) inhibition and elastase inhibition. S. chinensis was fermented with L. rhamnosus for 1 day at 37℃. The cytotoxicity of SCF was evaluated by a cytopathic effect reduction method. Effects on collagen biosynthesis and matrix metalloproteinase-I (MMP-1) of SCF were evaluated by previous reported method using procollagen type-IC peptide EIA kit and Matrix Metalloproteinase-1 Biotrack activity Assay Kit, respectively. Elastase inhibition assay was conducted by reaction of enzyme and substrate using N-Suc-(Ala)3-nitroanilide as the substrate. As the results, SCF didn't show cytotoxicity against human dermal fibroblast at concentration of 100μg/mL. Also, SCF was increased collagen synthesis and showed inhibitory effect of MMP-1 (p < 0.05). In the elastase inhibition assay, the IC50 of SCF was 36.4μg/mL. Therefore, our results indicated that SCF possesses anti-wrinkle effects and can be used practically for anti-wrinkle care of skin.
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KEYWORD
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Collagen biosynthesis, Matrix metalloproteinase-I, Schizandra chinensis Baillon fermented with Lactobacillus plantarum, Elastase, Anti-wrinkle
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